85-5989-27　FAM FLICA® Caspase 3/7 BioAssay™ Kit 100Tests　474006

特徴

• Sample Type:Cell culture
• Reagent Name:FAM-DEVD-FMK
• Target:Caspase-3/7
• Excitation/Emission:488nm/530nm
• Method of Analysis:Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader
• Intended Use:Detect caspase-3/7 activity with the FLICA Caspase-3/7 BioAssay™ Kit. This in vitro assay employs the fluorescent inhibitor probe FAM-DEVD-FMK to label active caspase 3 and 7 enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
• Test Principle:Caspases play important roles in apoptosis and inflammation. The FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FAM FLICA Caspase-3/7 assay probe allows researchers to assess caspase 3 and 7 activation.
• The FLICA reagent FAM-DEVD-FMK enters each cell and irreversibly binds to active caspases 3 and 7. Because the FAM-DEVD-FMK FLICA reagent becomes covalently coupled to the active enzymes, it is retained within the cell, while any unbound FAM-DEVD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining green fluorescent signal is a direct measure of the active caspase 3 and 7 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the kit. Unfixed samples may also be analyzed with Propidium Iodide or Hoechst 33342 to detect necrosis or changes in nuclear morphology, respectively.
• Kit Components:25 tests:FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 1 vial10X Apoptosis Wash Buffer, 1x15mlFixative, 1x6mlPropidium Iodide, 1x1mlHoechst 33342, 1x1ml100 tests:FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 4 vials10X Apoptosis Wash Buffer, 1x60mlFixative, 1x6mlPropidium Iodide, 1x1mlHoechst 33342, 1x1ml
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.