Intended Use:Quantify 8-OHdG in urine, cell culture, plasma and other sample matrices using DNA Damage (8-OHdG) ELISA BioAssay™ Kit. This BioAssay™ kit offers a quick incubation time, stable reagents, and a user-friendly protocol. Analyze results using an absorbance plate reader.
Test Principle:8-hydroxy-2-deoxyguanosine (8-OHdG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension. Quantify 8-OHdG in urine, cell culture, plasma and other sample matrices using DNA Damage ELISA BioAssay™ Kit. This BioAssay™ kit offers a quick incubation time, stable reagents, and a user-friendly protocol.
The ELISA utilizes an 8-hydroxy-2-deoxyguanosine-coated plate and an HRP-conjugated 8-OHdG specific detection antibody. This produces an BioAssay™ with a useful range of 0.94–60ng/mL, and a sensitivity of 0.59ng/mL. Analyze your results using an absorbance plate reader.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
