Intended Use:Assess assay feasibility and optimize ELISA performance parameters with Antigen-Down ELISA Development BioAssay™ Kit. The Antigen-Down ELISA Development Kit provides seven specially formulated ELISA Solutions and a template for the initial development and optimization of antigen-down immunoassays. This BioAssay™ kit is beneficial to both novice and experienced users. From coating buffer to substrate stop solution, this BioAssay™ kit contains enough reagent to construct ten 96-well ELISA plates.
Test Principle:An antigen-down enzyme-linked immunosorbent assay (AD ELISA) is an immunoassay-based technique that utilizes an ELISA plate coated with a target antigen to detect and/or quantify the presence of a specific antibody. Our AD ELISA Development BioAssay™ Kit provides seven specially formulated ELISA Solutions development components plus a detailed guide for the initial development and optimization process. AD ELISA formats are a preferred method for detecting antigen-specific antibodies that are present within serum, plasma, or cell culture supernatants (e.g., hybridoma screening). Everything from coating buffers to substrate and stop solutions, are included within this AD ELISA development BioAssay™ kit. Each BioAssay™ kit comes with enough liquid reagents and ELISA plates to perform ten 96-well ELISA plate BioAssay™, plus ten additional plates are included for performance of some optional steps (e.g. establishing a consensus dilution factor range for samples, performing a quality assessment of the plate coating process, normalizing AD ELISA OD450 signal variation events). Additional liquid components may be needed depending on the scale or scope of the individual AD ELISA development project.
This BioAssay™ kit is convenient, instructive and offers an economical pathway toward achieving your goal of developing your own research project specific, novel ELISA.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
