Intended Use:Assess BioAssay™ feasibility and optimize ELISA performance parameters with Antibody-Sandwich ELISA Development BioAssay™ Kit. Beneficial to both novice and experienced users, this kit provides eight specially formulated ELISA buffers, 96-well plates, plate storage materials, and a template for the initial development and optimization of a novel antibody-sandwich format ELISA. From coating buffer to substrate and stop solutions, this BioAssay™ kit contains enough reagent to perform ten 96-well ELISA plate BioAssay™.
Test Principle:An antibody-sandwich enzyme-linked immunosorbent assay refers to an ELISA plate configuration whereby the analyte being quantified is bound, or “sandwiched” between two layers of antibodies (i.e. capture and detection antibody). Antibody-sandwich ELISAs are used to quantify the amount of target analyte present in a sample. Antibody Sandwich ELISA Development BioAssay™ Kit provides eight specially formulated liquid components, 96-well plates, plate storage materials, plus a detailed guide for the initial development and optimization process of your own novel antibody-sandwich ELISA. Everything from coating buffer to substrate and stop solutions are included within this comprehensive development kit. Each kit comes with enough liquid reagent and ELISA plates to perform ten 96-well ELISA plate assays. This BioAssay™ kit is convenient, instructive, and offers an economical pathway toward achieving your goal of developing your own research project specific ELISA.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
