Fusion of a vesicle to its target membrane is a universal process in eukaryotic cells for proper cellular organization and function. Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes. SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca2+-dependent exocytosis. Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins. Munc18-1 acts as a molecular chaperone for syntaxin-1, allowing for formation of the SNARE complex at the plasma membrane. Following membrane fusion, the alpha-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the alpha-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion. The ATPase activity of NSF induces a conformational change in the alpha-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion, and eventual recycling of the SNARE complex for subsequent membrane fusion.
Specificity:Human
Intended Use:The Synaptic Vesicle Antibody Sampler BioAssay™ Kit provides an economical means of evaluating proteins involved in synaptic vesicle fusion and membrane trafficking. The kit contains enough primary and secondary antibodies to perform four western miniblot experiments with each antibody.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
