The Rho family of small GTPases, including Rho, Rac, and cdc42, act as molecular switches to regulate processes such as cell migration, adhesion, proliferation and differentiation. RhoA, RhoB and RhoC are all highly homologous but appear to have divergent biological functions. The best characterized of these proteins, RhoA, regulates acomysin contractility, cytokinesis, focal adhesion assembly and cell polarity. Mammalian Rac exists as three isoforms (Rac1, Rac2 and Rac3) that show high sequence similarity. Well-characterized Rac1 and cdc42 are ubiquitously expressed and play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, and cell growth and development. Phosphorylation of Rac1 at a putative Akt site (Ser71) may limit Rac1 activity through inhibition of GTP binding. Rac2 is expressed in cells of hematopoietic origin, while Rac3 is highly expressed in brain and in many other tissues. The Vav family of guanine-nucleotide exchange factors mediates activation of Rho/Rac family small GTPases. Negative regulation of Rho-activity members of the p190 RhoGAP family (p190-A and p190-B) may be controlled by Src phosphorylation of Tyr residues, activating the p190 GAP domain. Furthermore, Rho GDP dissociation inhibitor (RhoGDI) associates with Rho/Rac to negatively regulate nucleotide exchange membrane localization.
Specificity:Human
Intended Use:The Rho-GTPase Antibody Sampler BioAssay™ Kit contains reagents to examine aspects of cell migration, adhesion, proliferation and differentiation in cells. This kit includes enough primary and secondary antibodies to perform two Western blot experiments per each primary antibody.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
