Transcription factors of the nuclear factor kB (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-kB1 (p105/p50), and NF-kB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-kB is sequestered in the cytoplasm by IkB inhibitory proteins. NF-kB-activating agents can induce the phosphorylation of IkB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kB to enter the nucleus where it regulates gene expression. NIK and IKKalpha (IKK1) regulate the phosphorylation and processing of NF-kB2 (p100) to produce p52, which translocates to the nucleus).
Specificity:Human
Intended Use:This BioAssay™ kit contains reagents to examine total protein levels of the five NF-kB/Rel family members: p65/RelA, RelB, c-Rel, NF-kB1 (p105/p50) and NF-kB2 (p100). The kit includes enough primary and secondary antibody to perform two Western blot experiments with each antibody.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
