The NDRG (N-Myc downstream-regulated gene) family consisting of NDRG1, NDRG2, NDRG3, and NDRG4 are structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis. NDRG1 was originally identified as a protein that was upregulated in N-Myc knockout mice. Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues and have been suggested to function as a tumor suppressors. Ubiquitously expressed N-Myc downstream-regulated gene 1 (NDRG1) is highly responsive to a variety of stress signals, including DNA damage, hypoxia, and elevated levels of nickel and calcium. Expression of NDRG1 is elevated in N-Myc defective mice and is negatively regulated by N- and c-Myc. Expression of NDRG1 and NDRG2 is upregulated by p53 and HIF-1 and contributes to apoptosis driven by those pathways. Research studies show that NDRG1 may play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis. Nonsense mutation in the corresponding NDRG1 gene can cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival. NDRG1 is upregulated during mast cell maturation and its deletion leads to attenuated allergic responses. Elevated NDRG2 expression has been observed with Alzheimer's disease. Both NDGR1 and NDGR2 are phosphorylated at multiple sites by Akt and/or SGK1, although the precise physiological role of this phosphorylation is unclear. NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3. NDRG3 is most highly expressed in the testis, prostate, ovary, and brain and is upregulated by androgen in prostate cell lines, promoting cell growth in those cell lines. Unlike other widely expressed family members, several alternatively spliced NDRG4 (Bdm1) isoforms are expressed primarily in the heart and brain. Expression of NDRG4 is reduced in the brain of patients with Alzheimer's disease, but is elevated in glioblastoma where it contributes to cell cycle progression and survival. NDRG4 also may be inactivated by promoter methylation in colorectal cancer and function as a tumor suppressor gene.
Specificity:Human
Intended Use:The NDRG Family Antibody Sampler BioAssay™ Kit provides an economical means of detecting members of the NDRG protein family. The kit includes enough antibody to perform four western blot experiments per primary antibody.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
