The Mitochondrial Marker Antibody Sampler Kit contains a variety of antibodies directed against established mitochondrial proteins. Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane. Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to the mitochondrial intermembrane space. HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria. In the mitochondria, prohibitins (PHB1) mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation. In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix. Succinate dehydrogenase (SDH), also known as Complex II or succinate quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain. SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space. Voltage- dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria.
Specificity:Human
Intended Use:The Mitochondrial Marker Antibody Sampler BioAssay™ Kit provides an economical means to evaluate relevant mitochondial proteins. This kit contains enough primary antibody to perform two western blots per primary antibody.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
