Endosomes are formed by the invagination of the plasma membrane to form vesicles in an effort to recycle components of the cell. Endosomes can be coated in clathrin when vesicles form at clathrin-coated pits. Caveolins are 21-24kD integral proteins that interact with cholesterol and are the main structural components of the cholesterol/sphingolipid- enriched plasma membrane caveolae. Each stage of endosome maturation is marked by a unique set of proteins. EEA1 is an early endosome marker that is essential for membrane fusion and trafficking. Members of the ras superfamily of small Rab GTPases, specifically Rab5, Rab7, and Rab11 are markers of the early, late and recycling endosomes.
Specificity:Human
Intended Use:The Endosomal Marker Antibody Sampler BioAssay™ Kit provides an economical means of distinguishing endosomes in the early, late, and recycling phases. The kit includes enough antibody to perform two western blot experiments with each primary antibody.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
