Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through beta- and alpha-catenin. Research studies have demonstrated that loss of E-cadherin and alpha-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression. Research studies also suggest that, rather than acting as a static link between cadherins and actin, alpha-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex. alpha-catenin also plays a role in regulating beta-catenin- dependent transcriptional activity, affecting differentiation and response to Wnt signaling. alpha-catenin binds to beta-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation.
Specificity:Human
Intended Use:The Adherens Junction Antibody Sampler BioAssay™ Kit provides an economical means of detecting the protein components of adherens junctions. The kit includes enough antibody to perform four western blot experiments per primary antibody.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
