85-5981-86　Phospho-NPM (Thr199) BioAssay™ ELISA Kit 2x96Tests　473272

特徴

• The Phospho-NPM (Thr199) BioAssay™ ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NPM protein phosphorylation and expresssion profile in cells. It can be used for measuring the relative amounts of phosphorylated NPM in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on NPM phosphorylation.
• Sensitivity:>5000cells/well
• Kit Components:473272A: Microtiter Strips, 2x96 wells473272B: TBS, 10X, 1x24ml473272C: Quenching Buffer, 1x24ml473272D: Blocking Buffer, 1x50ml473272E: Wash Buffer, 15X, 1x50ml*473272F: NPM (Thr199) Pab, 100X, 1x60ul*473272G: NPM, 100X, 1x60ul473272H: GAPDH Mab, 100X, 1x60ul473272J: IgG Pab (HRP), 1x12ml473272K: IgG Mab (HRP), 1x12ml473272L: Primary Antibody Diluent, 1x12ml473272M: Substrate, 1x12ml473272N: Stop Solution (2N Sulfuric Acid), 1x12ml473272P: Crystal Violet Solution, 1x12ml*473272Q: SDS Solution, 1x24ml
• Storage and Stability:Store all components at 4°C. Reconstituted *473272F, *473272G and *473272H are stable for 2 weeks at 4°C. *473272Q should be stored at RT or warmed up to RT if stored at 4ºC. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.