Rheoviridae/Rotavirus/Rotavirus A/Simian rotavirus SA11. Virions consist of a capsid, a core, and a nucleoprotein complex. Virus capsid is not enveloped. Capsid/nucleocapsid is isometric with icosahedral symmetry and has a diameter of 80 nm. The genome is segmented and consists of eleven segments of linear double-stranded RNA. Rotavirus is the most common cause of severe diarrhea among children. The incubation period for rotavirus disease is approximately 2 days. The disease is characterized by vomiting and watery diarrhea for 3 - 8 days, and fever and abdominal pain occur frequently.
Source:Rotavirus, strain Simian rotavirus SA11, from MA104 cells.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:Rotavirus antigen is inactivated using gamma irradiation. This procedure is effective primarily by damaging viral genetic material. Rotavirus inactivation is assessed using a double-passage assay in tissue culture. Rotavirus antigen is inoculated onto an MA104 cell monolayer and incubated for three days. The infected culture is passaged and scored after a further 3 days of incubation for infectivity. The culture is monitored for cytopathiceffect. An absence of cytopathic affect, within the limit of the assay, indicates the inactivation of the antigen.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Source Antigen：MA104 cells
Grade：Purified
Purity：Infectious culture supernatant is collected and Rotavirus is purified from infected cells and the culture medium by selective precipitation. The precipitate is then pelleted by centrifugation and resuspended in buffer.
