This preparation contains more than 90% virus specific protein. The predominant form of the antigen is whole virions suspended in NTE with sucrose. The preparation is free of detergent. Residual non-viral protein comes from host tissue and culture medium.
Source:Highly purified (>99%) 125kD protein from P3H3 cells chronically infected with EBV.
Applications:Suitable for use in a variety of immunoassay formats including microwell ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:Detergent inactivation by lysis. Detergent treatment of P3H3 cells disrupts cellular and viral membranes and consequently renders the material non-infectious.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Source Antigen：P3H3 cells chronically infected with EBV.
Grade：Highly Purified
Purity：~99%. Chronically infected cells are harvested by centrifugation and disrupted by sonication and cell debris is removed by centrifugation. This lysate is purified on an immunoaffinity column using a monoclonal antibody against gp125.
Form：Supplied as a liquid in 20mM Tris-HCl, pH 7.4, 3M MgCl2.
