85-4785-23　［受注停止］DNA Ligase, Recombinant, Colwellia Psychrerythraea, aa330-689, His-tag (ligA) 20ug　405910

特徴

• DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
• Source:Recombinant protein corresponding to aa330-689 from Colwellia Psychrerythraea DNA Ligase, fused to His-tag, at N-terminal, expressed in E. coli.
• Molecular Weight:~42.1kD
• AA Sequence:KFPAEEELTCVEDVEFQVGRTGAITPVARLKPVFVGGVTVSNATLHNQDEITRLGLKVNDFVVIRRAGDVIPQIVSVVLDKRPDNAVDIVFPTSCPVCDSAVAKPEGEAVLRCTAGLFCAAQRKEAIKHFASRKAHDVDGLGDKLVEQLVDEKLINTPADLFKLTEIQVSTIDRMGKKSATNLINGLEQAKSTTLAKFIYGLGIREVGEATAANLANHFYTLAAIESASLEDLQNVSDVGEVVAKNIINFFKEEHNLAIVSGLSEVMHWPTIEIKSAEELPLAEQIFVLTGTLTQMGRTEAKTALQSLGAKVSGSVSKNTHFVVAGDKAGSKLTKAQDLGISVLTEDGLVALLAEHGITI
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.