Intended Use:This kit can be used for in vitro quantitative determination of zinc ion (Zn2+) in serum, plasma or urine.
Sensitivity:The actual limite of detection (LOD) is no more than 3.0umol/L.
Range:0~61.2umol/L, r2≥0.995
Test Principle:The Zn2+ in the sample can combine with 5-Br-PAPS in the reagent to form a colored complex at room temperature. The color of the complex is in directly proportional to the concentration of Zn2+ in the sample within the range of 400ug/dL.
Accuracy:Relative deviation≤15.0%
Precision: Intra-Assay CV: <4%Inter-Assay CV: <6%
Kit Components (see Protocol for final kit quantities):Reagent 1,Ascorbic acid, 50mmol/L4- (2-hydroxyethyl) -1-piperazine-ethanesulfonic acid (Hepes) buffer (pH 6.0), 200mmol/LTrisodium citrate (reaction enhancing solution), 0.2mol/L
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.