This kit applies the GPO-PAP method and it can be used for in vitro determination of triglyceride (TG) content. It can be operated with all kinds of spectrophotometer.
Range:0-9.04mmol/L, r2>0.995
Accuracy:Relative deviation ≤10%.
Precision: Intra-Assay CV: <5%Inter-Assay CV: <8%
Kit Components (see Protocol for final kit quantities):Working Solution (Enzyme), 100 mLx1 vial:Tris-HCL Buffer, 100mmol/LLipase, ≥3000U/LATP, 0.5mmol/LGlycerol Kinase, ≥1000U/LGlycerol-3- Phosphate Oxidase, ≥5000U/LPeroxidase, ≥1000U/L4-Aminoantipyrine, 1.4mmol/LParachlorphenol, 3mmol/L
Standard, 0.1 mLx1 vial:Glycerinum, 2.26mmol/L
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
