This kit applies the GPO-PAP method and it can be used for in vitro determination of triglyceride (TG) content. It can be operated with microplate reader, full-automatic or semi-automatic biochemistry analyzer.
Sensitivity:The absorbance value △A is between 0.2200~0.2900 when testing 2.26mmol/L samples.
Range:0-9.04 mmol/L, r2>0.995
Accuracy:Relative deviation ≤10%
Kit Components (see Protocol for final kit quantities):Working Solution (Enzyme), 25 mLx1 vial:Tris-HCL Buffer, 100mmol/LLipase, ≥3000U/LATP, 0.5mmol/LGlycerol Kinase, ≥1000U/LGlycerol-3-Phosphate Oxidase, ≥5000U/LPeroxidase, ≥1000U/L4-Aminoantipyrine, 1.4mmol/LParachlorphenol, 3mmol/L
Standard, 0.1 mLx1 vial:Glyceriunum, 2.26mmol/L
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
