Intended Use:This kit applies the COD-PAP method and it can be used for in vitro determination of total cholesterol (T-CHO) content. It can be operated with all kinds of spectrophotometer.
Range:0~10.34 mmol/L, r2>0.995
Precision: Intra-Assay CV: <3%Inter-Assay CV: <5%
Kit Components (see Protocol for final kit quantities):Work Solution (Enzyme), 100 mLx1 vial:Good’s Buffer, 50mmol/L, pH6.7Phenol, 5mmol/L4-AAP, 0.3mmol/LCholesterol esterase, >50KU/LCholesterol oxidase, >25KU/LPeroxidase, >1.3KU/L
Standard, 0.1 mLx1 vial:Cholesterol, 5.17mmol/L
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.