85-4783-88　［取扱停止］Total Bile Acid (TBA) BioAssay™ Kit 1Kit　405833

特徴

• The kit is used for the quantitative determination of the total bile acid concentration in serum. Total bile acid (TBA) is mainly used for the screening and prognosis of follow-up of hepatobiliary disease and as the marker of liver parenchymal damage and cholestasis. The increase of TBA indicates the risk of viral hepatitis, cirrhosis, alcoholic liver disease, drug-induced liver injury or cholestasis.
• Range:2.5~150umol/L, r2 ≥ 0.990.
• Test Principle:3alpha-hydroxycholic acid+NAD 3-Oxocholic+NADHdiaphorase NADH+INT Diaphorase NAD+formazane(red)Detect the absorbance at 505nm by colorimetry. The changes of A505 is proportional to the concentration of bile acid.
• Accuracy:Inaccuracy ≤ 15.0%; recovery rate ∈ (100 ± 20%).
• Precision: Intra-Assay CV: <5%Inter-Assay CV: <10%
• Kit Components (see Protocol for final kit quantities):Reagent 1, solution A, 36 mLx2 vial:Phosphate buffer, 100mmol/LDiaphorase, 1000U/LOxidized coenzyme I (NAD), 1mmol/LPyruvate, 50mmol/L
• Reagent 1, solution B, 4 mLx2 vial:Nitro-tetrazolium iodide (INT), 0.5mmol/L
• Reagent 2, 10 mLx2 vial:Phosphate buffer, 100mmol/L3alpha-hydroxysteroid dehydrogenase, 2000U/L
• Calibrator, 0.25 mLx1 vial:Sodium glycocholate, check the labelPreservative, 0.5g/L
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.