Sample Type:Animal serum, plasma, tissue, whole blood, red blood cells, culture cells, culture medium and other samples.
Intended Use:This kit can measure GSH content of all kinds of animal serum, plasma, tissue, whole blood, red blood cells, culture cells, culture medium and other samples.
Test Principle:Reduced glutathione (GSH) is a tripeptide which composed of glutamic acid, glycine and cysteine. It is a kind of low molecular scavenger, which can remove O2-, H2O2, and LOOH. Beside as the main thiol compound of non-protein in the organization, GSH is the substrate of GSH-PX and GST which is indispensable to decomposing hydrogen peroxide for the two enzyme. What’s more, it can stabilize the enzyme containing thiol and prevent hemoglobin and other auxiliary factors from the oxidative damage. Recently, it is proved that GSH is also involved in the recovery of vitamin E to the reduction state. When lacking or depletion of GSH, it may cause producing toxic effects or increasing the toxic effects of many chemicals or environmental factors. It may be related to the increase of oxidative damage, so the amount of GSH is a vital factor to measure the body's antioxidant ability.
Kit Components (see Protocol for final kit quantities):Reagent 1 A, Powderx1 vialReagent 1 B, 50 mLx1 vial
Reagent 2, Powderx1 vial
Reagent 3, 1xpowder
Reagent 4, 4xpowder
Reagent 5, 3x3.07 mg
Reagent6, 3 mLx1 vial
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
