The half-life of NO is extremely short, it exists in form of nitrate or nitrite produced by vascular endothelial cell, vascular smooth muscle cell, platelet, and macrophage and so on. The concentration of NO can be indirectly measured by detecting that of nitrate or nitrite. NO react with oxygen and water to generate nitrate or nitrite which can form a kind of pale red azo compound when meet with nitrate chromogenic reagent, the absorbance of the compound can be measured to calculate the concentration of NO indirectly.
Sample Type:Serum, plasma, tissue homogenates.
Kit Components (see Protocol for final kit quantities):Reagent 1, 20mL x 1vial:Liquid
Reagent 2, 10mL x 1vial:Liquid
Reagent 3, 10mL x 1vial:Liquid
Reagent 4, 3mL x 1vial:Liquid
Reagent 5, 3mL x 1vial:Liquid
Reagent 6, 1mL ×1vial:Sodium nitrite standard solution, 2mmol/L
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
