Maltase in the small intestine is a key enzyme of the carbohydrate absorption in the digestive tract. Maltase, sucrose and lactase are three important kinds of Maltase, which can respective hydrolyze maltose, sucrose and lactose into corresponding monosaccharide then be absorbed by the organism. If the organism lacks lactase or other Maltase, will lead to the disorder of carbohydrate digestion and absorption of food, the Undigested and Unabsorbed carbohydrates get into the large intestine, then be decomposed by bacteria into CO2, H2 and so on, give rise to diarrhea and other symptoms. Maltase acts on the substrate to produce monosaccharide, the monosaccharide produced oxidizing material by Oxidase action. The oxidizing material conjugate with chromogenic agent and the color turns red. The activity of maltase can be calculated by detection the optical density with spectrophotometer.
Sample Type:Animal tissue and other samples.
Intended Use:This kit can be used to detect the maltase activity in animal tissue and other samples.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Kit Components (see Protocol for final kit quantities)
