The body produce oxygen free radicals through the enzyme system and non-enzyme system, which can attack unsaturated fatty acid on biofilm, leading to lipid peroxidation and forming lipid peroxide, such as aldehyde group (MDA), keto group, hydroxyl group, carbonyl group etc. lipid peroxide can not only transform active oxygen to active chemical, but also enhance the effect of active oxygen. Detection of the content for MDA can reflect the level of lipid peroxidation in cells and reflect level of cellular damage indirectly.
Sample Type:Plant root, stem, leaves.
Intended Use:This kit can be used to measure the MDA content in different plant roots, stems, leaves and other samples.
Test Principle:The body produce oxygen free radicals through the enzyme system and non-enzyme system, which can attack unsaturated fatty acid on biofilm, leading to lipid peroxidation and forming lipid peroxide, such as aldehyde group (MDA), keto group, hydroxyl group, carbonyl group etc. lipid peroxide can not only transform active oxygen to active chemical, but also enhance the effect of active oxygen. Detection of the content for MDA can reflect the level of lipid peroxidation in cells and reflect level of cellular damage indirectly.
Kit Components (see Protocol for final kit quantities):Reagent 1, 3mL × 1vial:Clarificant
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.