The kit is used for auxiliary diagnosis of related diseases by determining the serum homocysteine concentration. Homocysteine is mainly used as a risk indicator of cardiovascular disease, especially coronary atherosclerosis and myocardial infarction. The increase in homocysteine concentration is proportional to the risk of disease and is an independent risk factor to induce cardiovascular disease.
Range:0-50umol/L, r2 ≧ 0.990
Test Principle:Oxidized homocysteine (Hcy) is reduced to free homocysteine, and the free homocysteine reacts with S-adenosylmethionine (SAM) under the catalysis of homocysteine-methyltransferase to generate methionine and S-adenosylmethionine (SAH). SAH is hydrolyzed by SAH hydrolase to generate adenosine and Hcy. Then the circular reaction start when the formed Hcy is involved in the Hcy-Methyltransferases. The cyclic reaction result in a significant amplification of the detection signal. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD. The decrease in absorbance at 340nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
