H+, K+-ATPase has important physiological significance in gastric acid secretion and gastric digestive function. Forte et al first isolated a class of ATPase in bullfrog gastric mucosa that can be specific activated by K + and can't inhibited by ouabain, in the 70's. It is H+、K+-ATPase. H + K + -ATPase located in the cell of Gastric mucosa wall, belong to the second class of proton pump. Through its phosphorylation (E1→E2) and dephosphorylated (E2→E1) to complete the H + /K + electric neutrality transmembrane ion transport, constantly transport proton within the wall cell to outside the cell membrane to perform acid secretion function.
Kit Components (see Protocol for final kit quantities):Reagent 1,Liquid, 10 mLx3 vial
Reagent 2,Liquid, 7 mLx2 vial
Reagent 3,Liquid, 8 mLx2 vial
Reagent 4,Powder, 4xvial
Reagent 5,Powder, 4xvial
Reagent 6,Liquid, 3 mLx2 vial
Reagent 7,Liquid, 10 mLx2 vial
Reagent 8,Powder, 5xvial
Reagent 9,Powder, 2xvial
Reagent 10,Sulfure acid 2.5moL/L, 100 mLx2 vial
Reagent 11,Standard 10mmol/L, 10 mLx1 vial
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
