Glutathione Peroxidase (GSH-PX) is an important enzyme that catalyzes decomposition of hydrogen peroxide. GSH specifically catalyze the reaction between GSH and hydrogen peroxide, protecting cell membrane structure and keeping membrane function integrity. Se-cysteine is the active center of the GSH-PX. Determination of GSH-PX activity in organism can be an indicator of selenium level as Se is essential section of GSH-PX.
Sample Type:Serum, plasma, tissue, whole blood, red blood cell
Intended Use:This kit can be used to measure GSH-PX activity of animal serum, plasma, tissue, whole blood, red blood cell and other samples.
Kit Components (see Protocol for final kit quantities):Reagent 1,Sample homogenate, 50mL
Reagent 2,GSH, 15.4mg
Reagent 3,GSH preparation solution, 10mL
Reagent 4,Oxidant, 2×1 mL
Reagent 5,Acidic precipitation agent, 50mL
Reagent 6,GSH-Px assay buffer, 15mL
Reagent 7,Benzoic acid chromogenic solution, 3mL
Reagent 8,ddH2O, 50mL
Manual, 1xcopy
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
