Gamma-glutamylcysteine Synthetase (Gamma-GCS) is the rate-limiting enzyme in the synthesis of glutathione (GSH). Intracellular Gamma-GCS activity is increased with the acceleration of GSH synthesis, thereby increasing the concentration of intracellular and extracellular GSH. GSH is an important antioxidant in the body. GSH plays a significant role in maintenance of airway epithelial cell integrity, resistance to lung injury and inflammation and antioxidant damage. The activity of Gamma-GCS and the concentration of GSH (6.1~17.5nmol/mg lung tissue) are both high in the lung. The concentration of GSH in the lining of respiratory tract epithelium (200~400umol/L) is 100 times higher than the one in the plasma. Thus GSH plays an essential role in respiratory inflammation and allergic reactions; and GSH has great research significance in the treatment and prognosis of cardiovascular disease and tumor.
Kit Components (see Protocol for final kit quantities):Reagent 1,Buffer, 1x25mlReagent 2,Substrate agent, 1x25mlReagent 3,Accelerant agent, 3xpowder, solution 1x10mlReagent 4,Termination, 1x6mlReagent 5,Chromogenic agentSolution A, 8x7mlSolution B, 8x6mlReagent 6,Stabilizing agent, 1x100ml Reagent 7,Standard phosphate stock solution 10mmol/L, 1x5ml
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
