Intended Use:This kit can be used to detect the CRE content in animal serum, plasma, urine and other samples.
Test Principle:Creatinine (CRE) can be catalyzed by creatinine amide hydrolysis and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatine amino hydrolase, the sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4-(6-Tri-iodine-3- hydroxybenzoic acid) and 4-aminoatipyrine can be catalyzed by peroxidase and form purple complex. The result can be colorimetric determined at 546nm.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Kit Components (see Protocol for final kit quantities)
