Intended Use:This kit can be used for in vitro quantitative determination of coper ion (Cu2+) in serum and plasma.
Sensitivity:The actual limit of detection (LOD) is no more than 1.5umol/L.
Range:0~78.7umol/L, r2≥0.995
Test Principle:The Cu2+ in the sample can dissociate from copper-protein and combine with 3,5-dibromo-PAESA to form an amaranth complex in pH5.0 buffer. The absorbance is in proportional to the concentration of Cu2+ in certain range.
Accuracy:Relative deviation ≤15.0%
Precision: Intra-Assay CV: <4%Inter-Assay CV: <6%
Kit Components (see Protocol for final kit quantities):Reagent 1,Acetate buffer pH5.0, 0.2mol/LSodium deoxycholate (SDC), 2×10-2mol/L
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
