Sample Type:Animal tissue, serum, plasma, whole blood and cultured cells, and cell culture supernatant.
Intended Use:This kit can be used for detection of Acetylcholinesterase (A-CHE) activity in animal tissue, serum, plasma, whole blood and cultured cells, and cell culture supernatant.
Kit Components (see Protocol for final kit quantities):Reagent 1,Powder, 3xvial
Reagent 2,Powder, 2xvialPreparation of Substrate buffer: Add 10 mL of normal saline into 1 vial of Reagent 2 Powder, mix fully. Thee prepared Substrate buffer can be stored at 4°C for 2 weeks.
Reagent 3,Liquid, 3 mLx1 vialPreparation of Chromogenic agent application solution: Dilute the stock solution with normal saline at 1:9. Prepare the needed amount. Or prepare 30 mL at one time and store it at 4°C in the dark.
Reagent 4,Liquid, 2 mL×1 vial
Reagent 5,Liquid, 6 mLx1 vial
Normal saline, 60 mLx2 vial
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
