5'-NT is widely distributed in the liver, biliary system and various organs and tissues. The enzyme activity of 5'-NT in serum increased (2 ~ 6) mainly in the biliary system diseases, such as obstructive jaundice, primary and secondary liver cancer, and it usually parallel with the activity changes of ALP.
Sample Type:Animal serum, plasma, tissue
Intended Use:This kit can be used for detection of 5’-Nucleotidase (5’-NT) activity in animal serum, plasma, tissue and other samples.
Test Principle:5’-NT can catalyze the hydrolysis of Adenosine -5- phosphate and produce adenosine and inorganic phosphorus. 5’-NT activity can be calculated by detecting the content of inorganic phosphorus. The influence of other enzymes can be inhibited by adding inhibitor.
Kit Components (see Protocol for final kit quantities):Reagent 1,Liquid, 80 mLx1 vial
Reagent 2,Liquid, 12 mLx1 vial
Reagent 3,Liquid, 6 mLx1 vial
Reagent 4,Powder, 2xvialDiluent, 6 mLx2 vial
Reagent 5,Liquid, 60 mLx2 vial
Reagent 6,Liquid, 50 mLx1 vial
Reagent 7,Powder, 1xvial
Reagent 8,Powder, 1xvial
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
