85-4782-89　［取扱停止］Zika virus infected Vero cell lysate, Uganda MP1751 100ug　397652

特徴

• Zika virus is an emerging disease that is spread by Aedes mosquitoes. The virus was first isolated in Central Africa, and has since been spread to South Asia and recently to South America. Outbreaks were reported in Micronesia in 2007 and in Brazil in 2015, confirming at least 13 autochthonous infections. The Zika virus outbreak in Brazil in 2016 has gained world-wide attention, and has been linked to an increasing number of microcephaly cases and Guillain-Barre syndrome. In April 2016 the Centers for Disease Control, in the USA, confirmed the link between Zika virus infection of the fetus with microcephaly. Zika virus can cause mild fever, rash, myalgia, arthralgia and headaches, with one in four infected individuals being asymptomatic. Due to similar symptoms Zika virus infected individuals can easily be mis-diagnosed as an infection with other arboviruses, such Dengue, Chikungunya and Oropouche. In addition, Zika virus has been implicated in causing microcephaly through transmission in utero. There is no vaccine or specific treatment available for Zika virus. There is currently a major worldwide effort underway to develop effective diagnostic assays that can both detect Zika virus infection, and distinguish infection from other flaviviruses, especially Dengue virus infection.
• The lysate was clarified by centrifugation and was heat inactivated. ELISA analysis using human sera have shown that this lysate is recognized only by anti-Zika IgG and not anti-dengue-IgG antibodies in human serum.
• Description:Vero cells infected with Zika virus were harvested 7 days post infection, and collected cell debris was lysed in PBS containing 1% Triton X-100. Then, lysate was cleared by centrifugation and heat inactivated at 56°C for 1hr.
• Inactivation:Virus could not be cultured from 1ul of lystate as determined by infection assay. However, lystate should be handled under BSL 2 conditions since the presence of infectious virus cannot be excluded.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.