Dengue virus serotype 4 Envelope protein (E protein) has been manufactured in response to the unmet need for highly purified, concentrated protein for use in serological based diagnostic assays. Dengue virus serotype 4 Envelope protein is engineered in human cells using state-of-the-art expression and purification techniques. The soluble domain of the Dengue virus serotype 4 Envelope protein is expressed in HEK293 cells, and purified by IMAC and IEX chromatography. Dengue virus is a member of the flavivirus family, which includes Zika virus, West Nile virus and Japanese Encephalitis virus. Many of the proteins expressed by these viruses are very similar, and serological testing for these viruses can be complicated by problems of cross-reactivity. In this context, the use of highly purified antigens, that are glycosylated and folded as native proteins can be highly important in the development of accurate immunoassays
With more than one-third of the world’s population living in areas at risk of transmission, dengue infection is a leading cause of illness and death in the tropics and subtropics. As many as 100 million people are infected yearly. Dengue is caused by any one of four related viruses transmitted by mosquitoes. It is a febrile illness that affects infants, young children and adults with symptoms appearing 3-14 days after the infective bite. Symptoms range from mild fever, to incapacitating high fever, with severe headache, pain behind the eyes, muscle and joint pain, and rash. There is no vaccine or any specific medicine to treat dengue. Severe dengue (also known as dengue hemorrhagic fever) is characterized by fever, abdominal pain, persistent vomiting, bleeding and breathing difficulty and is a potentially lethal complication, affecting mainly children. Early clinical diagnosis and careful clinical management by trained physicians and nurses increase survival of patient.
Recombinant protein corresponding to aa280-678 of Dominica/814669/1981 strain of Dengue virus serotype 4 Envelope protein, fused to His-Tag at C-terminal, expressed in 293 human cells
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, 293 human cells
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a liquid in 20mM Tris-HCl, pH 7.8, 110mM sodium chloride.
