This Crimean Congo hemorrhagic fever (CCHF) virus is a tick-borne enveloped single stranded RNA virus that belongs to the genus Nairovirus and a member of the Bunyaviridae family. CCHF virus causes a hemorrhagic disease in humans with up to 80% case fatality. Although the virus has only caused sporadic disease in the past, the expansion of the range of its vector, the Hyalomma tick, is causing increasing concern that case numbers will continue to rise. Since CCHF was first described in Crimea in 1944, sporadic outbreaks have occurred globally. In 2015, CCHFV was identified by the WHO as an emerging virus which is likely to cause a severe epidemic and which may present a public health emergency.
Zoonotic transmission from host animals is the primary route of infection, with concerns this may increase in the next 10-20 years as the timing of religious festivals change. The virus may also be spread effectively by human to human contact, especially in hospital settings. There are also concerns that it may be used as a bioterrorism agent, with evidence that work on this was carried out both in the USSR and in Iraq. Research into diagnosis of the illness, and also into vaccine development is increasing. The CCHF virus Glycoprotein N is a potential target for vaccine development, as studies have shown that neutralizing antibodies may be raised against this protein. Studies on the Gn protein have shown that correct glycosylation is important for for the function of the protein.
Recombinant protein corresponding to Crimean Congo Hemorrhagic Fever CCHF Virus Glycoprotein N, strain IbArl0200, aa520-690, corresponding to full length glycoprotein precursor of the M segment of CCHFV, the transmembrane region deleted, and replaced with a 6xHis tag at C-Terminal, expressed in mammalian cells.
Molecular Weight:~25-30kD
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, Mammalian cells
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a liquid in 10mM Tris-HCl, pH 7.8, 125mM sodium chloride.
