Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor B (NFB). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23aa and a mature peptide of 76aa. It is a monomeric polypeptide, with a molecular weight of ~13kD. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5.
Recombinant protein corresponding to CCL2 expressed in E. coli.
Molecular Weight:~8.7kD
Biological Activity:CCR2 activity is verified by intracellular calcium flux using THP1 cells as described by Veldkamp et. al, Science Signaling 2008.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥98% (SDS-PAGE, HPLC, MALDI-MS and NMR Analysis). Processed, refolded and purified to yield the native, secreted form of the mature CCL2.
Form：Supplied as a lyophilized powder in salt-free acetonitrile, water. Reconstitute with sterile dH2O.
