RPS6KB1, also known as ribosomal protein S6 kinase beta-1 isoform a, is a serine/threonine kinase that acts downstream of PIP3 and phosphoinositide-dependent kinase-1 in the PI3 kinase pathway. Phosphorylation of S6 induces protein synthesis at the ribosome. The phosphorylation of p70S6K at threonine 389 has been used as a hallmark of activation by mTOR and correlated with autophagy inhibition in various situations. However, several recent studies suggest that the activity of p70S6K plays a more positive role in the increase of autophagy. Recombinant human RPS6KB1, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques.
Source:Recombinant protein corresponding to aa1-525 of human RPS6KB1, fused to His-Tag at C-terminal, expressed in insect cell.
Molecular Weight:~60.2kD (533aa), ~70-100kD (SDS-PAGE under reducing conditions.)
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, Insect cell
Grade：Purified
Purity：~85% (SDS-PAGE)
Form：Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 2mM DTT, 0.1mM PMSF, 0.5M sodium chloride, 30% glycerol.
