85-4781-55　NNMT, Recombinant, Human, aa1-264, His-Tag (Nicotinamide N-methyltransferase) 100ug　396912

特徴

• NNMT(Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-Tag at N-terminal, expressed in E. coli and purified by using conventional chromatography.
• Source:Recombinant protein corresponding to aa1-264 of human NNMT, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~37.7kD (284aa), confirmed by MALDI-TOF
• Endotoxin:<1EU/1ug (determined by LAL method)
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL
• Specific Activity: >100nmol/min/mg, and is defined as the amount of enzyme that transfer 1nmole of methyl group/minute at 37°C.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.