85-4781-47　Mdh2, Recombinant, Mouse, aa25-338, His-Tag, (Malate Dehydrogenase 2, MDH, Mdh-2, Mor-1, Mor1) 100ug　396906

特徴

• Mdh2, is also known as Malate dehydrogenase 2. Mdh2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, Mdh2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant mouse Mdh2 protein, fused to His-Tag at N-terminal, expressed in E. coli and purified by using conventional chromatography techniques.
• Source:Recombinant protein corresponding to aa25-338 of mouse Mdh2, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~35.4kD (335aa), Confirmed by MALDI-TOF
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK
• Specific Activity:>800units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD/minute at pH 8.0 and 37°C.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.