85-4781-21　IFNG, Recombinant, Human, aa24-161, His-Tag (Interferon Gamma, IFG, IFI, Interferon Gamma) 100ug　396882

特徴

• IFNG, also known as Interferon gamma, is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Nature human interferon-gamma is composed of 143aa residues without cysteine residues and is glycosylated. Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. Recombinant human IFNG protein, fused to His-Tag at N-terminal, expressed in E.coli and purified by using conventional chromatography techniques.
• Source:Recombinant protein corresponding to aa24-161 of human IFNG protein, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~18.4kD (159aa), Confirmed by MALDI-TOF
• Endotoxin:<1EU/1ug (determined by LAL method)
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG
• Biological Activity:Measured in a cytotoxicity assay using WiDr cells. The ED50 for this effect is less or equal to 5ng/ml.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.