85-4781-10　GZMB, Recombinant, Human, aa19-247, His-Tag (Granzyme B Isoform 1, GZMB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT) 20ug　396872

特徴

• GZMB, also known as granzyme B isoform 1, is member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. Tis protein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. Also, it processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. Recombinant human GZMB, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques.
• Source:Recombinant protein corresponding to aa19-247 of human GZMB, fused to His-Tag at C-terminal, expressed in insect cell.
• Molecular Weight:~26.5kD (235aa), ~28-40kD (SDS-PAGE under reducing conditions.)
• Endotoxin:<1EU/1ug (determined by LAL method)
• AA Sequence:GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH
• Biological activity:Specific activity is >7,000pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37°C.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.