85-4780-56　Ak1, Recombinant, Mouse, aa1-210, His-Tag (Ak-1, Adenylate Kinase Isoenzyme 1 Isoform 1) 5ug　396827

特徴

• Ak1, also known as Adenylate kinase isoenzyme 1 isoform 1, is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes.It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in Ak1 are the cause of a form of hemolytic anemia. Recombinant mouse Ak1 protein, fused to His-Tag at N-terminal, expressed in E. coli and purified by using conventional chromatography techniques.
• Source:Recombinant protein corresponding to aa1-210 of mouse Ak1 protein, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~25.5kD (233aa), Confirmed by MALDI-TOF
• Endotoxin:<1EU/1ug (determined by LAL method)
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSMGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK
• Specific Activity:>150units/mg. One unit will convert 2umoles of ADP to ATP + AMP/minute at pH 7.5 at 37°C.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.