85-4780-52　Adenosine Kinase, Recombinant, Human, aa22-362, His-Tag (ADK, AK) 20ug　396824

特徴

• Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant human Adenosine kinase, fused to His-Tag at N-terminal, expressed in E. coli and purified by using conventional chromatography techniques.
• Source:Recombinant protein corresponding to aa22-362 of human Adenosine kinase, fused to His-Tag at N-terminal, expressed in E. coli.
• Molecular Weight:~40.5kD (362aa), Confirmed by MALDI-TOF
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD FH
• Specific Activity:>30pmol/min/ug and is defined as the amount of enzyme that convert 1pmole of adenosine to AMP/minute at pH 7.5 at 37°C in a couple system with PK and LDH.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.