85-4779-33　PD-L1/B7-H1 BioAssay™ ELISA Kit (Mouse) 96Tests　396739

特徴

• Programmed death-ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2.
• Sample Type:Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA).
• Intended Use:Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Mouse PD-L1/B7-H1.
• Sensitivity:<10pg/ml
• Range:31.2pg/ml-2000pg/ml
• Specificity:Recognizes natural and recombinant Mouse PD-L1/B7-H1. There is no detectable cross-reactivity with other relevant proteins.
• Test Principle:This Mouse PD-L1/B7-H1 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for PD-L1/B7-H1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: F19-H239) and test samples are added to the wells, a biotinylated detection specific for PD-L1/B7-H1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PD-L1/B7-H1 amount of sample captured in plate.
• Kit Components:96-well plate precoated with anti-Mouse Cd274 antibody, 1x plateLyophilized recombinant Mouse Cd274 standard, 2x10ng/tubeBiotinylated anti-Mouse Cd274 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x buffer
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.