85-4778-69　GDF5 BioAssay™ ELISA Kit (Rat) 96Tests　396675

特徴

• Growth/differentiation factor 5 is a protein that in humans is encoded by the GDF5 gene. GDF5 is a protein belonging to the transforming growth factor beta superfamily that is expressed in the developing central nervous system, and has a role in skeletal and joint development. It also increases the survival of neurones that respond to the neurotransmitter dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.
• Sample Type:Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA).
• Intended Use:Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Rat GDF5/Bmp 14.
• Sensitivity:<10pg/ml
• Range:31.2pg/ml-2000pg/ml
• Specificity:Recognizes natural and recombinant Rat GDF5/Bmp 14. There is no detectable cross-reactivity with other relevant proteins.
• Test Principle:This Rat GDF5/Bmp 14 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for GDF5/Bmp 14 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A376-R495) and test samples are added to the wells, a biotinylated detection specific for GDF5/Bmp 14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat GDF5/Bmp 14 amount of sample captured in plate.
• Kit Components:96-well plate precoated with anti-Rat Gdf5 antibody, 1x plateLyophilized recombinant Rat Gdf5 standard, 2x10ng/tubeBiotinylated anti-Rat Gdf5 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x buffer
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.