85-4778-30　EGFR BioAssay™ ELISA Kit (Mouse) 96Tests　396636

特徴

• The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.
• Sample Type:Cell lysates, cell culture supernates, serum, plasma (heparin or EDTA).
• Intended Use:Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Mouse EGFR.
• Sensitivity:<15pg/ml
• Range:156pg/ml-10,000pg/ml
• Specificity:Recognizes natural and recombinant Mouse EGFR. There is no detectable cross-reactivity with other relevant proteins.
• Test Principle:This Mouse EGFR BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for EGFR has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: L25-S647) and test samples are added to the wells, a biotinylated detection specific for EGFR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse EGFR amount of sample captured in plate.
• Kit Components:96-well plate precoated with anti-Mouse Egfr antibody, 1x plateLyophilized recombinant Mouse Egfr standard, 2x10ng/tubeBiotinylated anti-Mouse Egfr antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x buffer
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.