85-4778-29　EDIL3 BioAssay™ ELISA Kit (Human) 96Tests　396635

特徴

• EDIL3, also known as DEL1, is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellular matrix. It is regulated by hypoxia or vascular injury and has been implicated in vascular remodeling during angiogenesis. The EDIL3 gene is mapped to 5q14. Human endothelial cells bind to Del1 together with the alpha-V (ITGAV)/beta-3 (ITGB3) integrin receptor. It is confirmed that adhesion of endothelial cells to Del1 is integrin mediated and that Del1 binds ITGAV/ITGB3. Functional analysis showed that both Del1 and vitronectin (VTN) stimulate endothelial cell migration. Immunoprecipitation and immunoblot analyses revealed that adhesion of endothelial cells to Del1 induces tyrosine phosphorylation of PTK2.
• Sample Type:Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA).
• Intended Use:Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Human EDIL3.
• Sensitivity:<10pg/ml
• Range:15.6pg/ml-1000pg/ml
• Specificity:Recognizes natural and recombinant Human EDIL3. There is no detectable cross-reactivity with other relevant proteins.
• Test Principle:This Human EDIL3 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for EDIL3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: D24-E480) and test samples are added to the wells, a biotinylated detection specific for EDIL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EDIL3 amount of sample captured in plate.
• Kit Components:96-well plate precoated with anti-Human EDIL3 antibody, 1x plateLyophilized recombinant Human EDIL3 standard, 2x10ng/tubeBiotinylated anti-Human EDIL3 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x buffer
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.