85-4778-10　CD56/NCAM-1 BioAssay™ ELISA Kit (Monkey) 96Tests　396616

特徴

• Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
• Sample Type:Cell culture supernates, serum, plasma (heparin or EDTA).
• Intended Use:Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Monkey CD56/NCAM-1.
• Sensitivity:<10pg/ml
• Range:156pg/ml-10,000pg/ml
• Specificity:Recognizes natural and recombinant Monkey CD56/NCAM-1. There is no detectable cross-reactivity with other relevant proteins.
• Test Principle:This Monkey CD56/NCAM-1 BioAssay™ ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey CD56/NCAM-1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: L20-G718) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey CD56/NCAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 215 Monkey CD56/NCAM-1 amount of sample captured in plate.
• Kit Components:96-well plate precoated with anti-Monkey NCAM1 antibody, 1x plateLyophilized recombinant Monkey NCAM1 standard, 2x10ng/tubeBiotinylated anti-Monkey NCAM1 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x buffer
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.