85-4777-95　Cadherin-2 BioAssay™ ELISA Kit (Porcine) (N-Cadherin) 96Tests　396602

特徴

• Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
• Sample Type:Cell culture supernates, serum, plasma (heparin or EDTA).
• Intended Use:Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Porcine Cadherin-2/N-Cadherin.
• Sensitivity:<20pg/ml
• Range:0.78ng/ml-50ng/ml
• Specificity:Recognizes natural and recombinant Porcine Cadherin-2/N-Cadherin. There is no detectable cross-reactivity with other relevant proteins.
• Test Principle:This Porcine Cadherin-2/N-Cadherin BioAssay™ ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Porcine Cadherin-2/N-Cadherin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Porcine Cadherin-2/N-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine Cadherin-2/N-Cadherin amount of sample captured in plate.
• Kit Components:96-well plate precoated with anti-Porcine CDH2 antibody, 1x plateLyophilized recombinant Porcine CDH2 standard, 2x50ng/tubeBiotinylated anti-Porcine CDH2 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x buffer
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.