85-4777-49　Afamin BioAssay™ ELISA Kit (Human) 96Tests　396555

特徴

• Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.
• Sample Type:Cell culture supernates, serum, urine.
• Intended Use:Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Human Afamin.
• Sensitivity:<10pg/ml
• Range:31.2pg/ml-2000pg/ml
• Specificity:Recognizes natural and recombinant Human Afamin. There is no detectable cross-reactivity with other relevant proteins.
• Test Principle:This Human Afamin BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Afamin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L22-N599) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Afamin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Afamin amount of sample captured in plate.
• Kit Components:96-well plate precoated with anti-Human AFM antibody, 1x plateLyophilized recombinant Human AFM standard, 2x2ng/tubeBiotinylated anti-Human AFM antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x buffer
• Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.